Determination of glutathione stransferase activity in crude homogenate of maize weevil (Sitophilus Zeamais) under basal metabolic condition Department: Biochemistry By: sagiersageer Project ID: 6874 Rating: (3.7) votes: 3Rate this project12345 Price:₦4000 Get the Complete MaterialAbstractGlutathione S-transferases (GSTs) are bisubstrate enzymes belonging to the phase II metabolism that catalyze the formation of thioether conjugates between the endogenous tripeptide glutathione and xenobiotic compounds. Glutathione S-transferases Total activity of the maize weevil (Sitophilus Zeamais) GSTswere Determined for Two substrates, 1-chloro-2,4-dinitrobenzene (CDNB) and Ethacrynic acid. The result of total activity showed that CDNB has (0.269µmol/mL.min)while EA (3.8µmol/mL.min). The kinetic parameters were determined and the Michaelis constant (Km) was calculated for both CDNB and GSH. The Km values using CDNB (6.547266µM) were higher as compared to that of GSH (0.603398µM). The data indicate the presence of the enzymes in the maize weevil but the enzyme has lower affinity to the CDNB when compared with GSH. This showed that these enzymes might play a role in the detoxification of insecticides and may possibly contribute to the development of insecticide resistance. The insect was able to detoxify the xenobiotics (CDNB) at a faster rate than their endogenous substrate (GSH)....Preview Download Preview +Other Biochemistry project topics and materials you might be interested in»Phytochemical and nutritive composition of fluted pumpkin»Determination of ascorbic acid in selected fruits mango, orange and pineapple using spectro-photometric method»Comparative evaluation of heavy metal loads in some selected soft drinks in Owerri, Imo state»Antilipidemic effect of water (H2O) extract of desmodium velutinum leaves on albino Wistar rats»The effect of water extract of cola nitida pod on lipoprotein concentrations of albino Wistar rats»Preliminary investigation on effects of burantashi extract on lipoproteins of albino male and female whistar rats»The extraction of manganese (iv) ion from aqueous media using 1-phethyl-3-methyl pyrazolone-5»Nutritive value, anti-nutrient and mineral composition of cashew nut (Anacardium Occidentale)»Evaluation of the immunomodulatory effect of oral administration of the methanol extract of gongronema latifolium (utazi) in wistar albino rats»Comparative study on the free radical scavenging potential of turmeric and beetroot»Phytochemicals and minerals analysis of rosary pea (Abrus Precatorious leaves)»Determination of total petroleum hydrocarbons in Niger river»Qualitative phytochemical and gas chromatography mass spectrometry GCMS of Nhexane extracted leaves of sabicea calycina»Hydrogen peroxide scavenging activities and reducing power of various fruits juice in Yenagoa»Charges in the concentration of nitric oxide in the serum gills brain liver and kidney of clarias gariepinus exposed to glyphosatebased herbicide